Affymetrix Analytical Services and Pricing
GeneChip® probe arrays are manufactured through a unique and robust process, a combination of photolithography and combinational chemistry. With a minimum number of synthesis steps, the technology produces arrays with hundreds of thousands of different probes packed at an extremely high density enabling researchers to obtain high quality, genome-wide data using small sample volumes. (Images courtesy of Affymetrix.)
The MCF processes RNA and DNA samples for use on the Affymetrix GeneChip® platform. Prices and services are shown below for Baylor clients.
| SAMPLE QUALITY SERVICES | COST** |
|---|---|
| Sample Quality Control (Diluted) PicoChip - One small concentration (< 25 ng/µl) sample tested for quality assessment on the Agilent 2100 Bioanalyzer using the Lab-On-A-Chip Technology and the Nanodrop ND-1000 Spectrophotometer. Please bring samples during normal sample drop-off times. | $20/sample |
| Sample Quality Control (Diluted) NanoChip - One sample (between 25 ng/µl and 500 ng/µl) tested for quality assessment on the Agilent 2100 Bioanalyzer using the Lab-On-A-Chip Technology and the Nanodrop ND-1000 Spectrophotometer. Please bring samples during normal sample drop-off times. | $15/sample |
| Sample Quality Control Genomic DNA- One sample (50 ng/nl or greater) for use on the Affymetrix SNP Mapping Arrays (10K, 100K set, 500K set). Please bring samples during normal sample drop-off times. | $15/sample |
| Sample QC (Non-Diluted) - there is an additional charge if the MCF is required to dilute your sample. | $15/sample |
| Sample QC Printed Report - Report of RNA Quality of all samples done in a single day, pulled together, printed, email notification, and delivery with explanation. | $15/request |
| Sample QC Electronic Report - This is the RNA QC report electronically available to the user through MAID. | $5/request |
| GENE EXPRESSION/RNA ANALYSIS SERVICES | COST** |
| Affymetrix Labeling (Total RNA) - This service includes time, reagents, cleanup, and QC for RT, 2nd strand synthesis, IVT with biotin, fragmentation, of 5.0 µg of total RNA. | $340/sample |
| Affymetrix Small Sample Prep and Labeling (Total RNA) - This service includes time, reagents, cleanup, and QC to amplify a user's limited RNA sample using either the Affuymetrix Two-Cycle Labeling Kit or the Nugen Ovation Labeling kit. Affymetrix kit uses between 10 and 100 ng Total RNA and the Nugen kit uses 5.0 ng-100.0 ng total RNA. | $460/sample |
| SNP GENOTYPING/DNA ANALYSIS SERVICES | COST** |
| Affymetrix Labeling (Genomic DNA) - This service includes time, reagents, cleanup, and QC for the restriction digest, ligation, PCR, fragmentation, and end-labeling, of 0.25 µg of genomic DNA. Visit the Affymetrix page on DNA Analysis | $230/sample/chip |
| HYBRIDIZATION & SCANNING SERVICES | COST** |
| Affymetrix Hybridization - Hybridization services using our control spikes, hybridization solutions and cocktail to load users' sample(s) on to a GeneChip® or the Mapping 10K Array. | $75/array |
| Affymetrix Washing/Staining on the Fluidics Station - Preparing a GeneChip® or a Mapping SNP Array for washing and staining with SAPE after hybridization. | $125/array |
| Laser Scanning on the GeneChip Scanner 3000 - Scanning a single GeneChip® or a Mapping SNP Array on the Affymetrix GeneChip® Scanner 3000. | $25/array |
** Support funding from NIH center grants allows the MCF to offer reduced pricing to certain investigators who qualify. Please contact the MCF to inquire about reduced pricing.
NOTE: Users wishing to submit aRNA (amplified RNA) need to READ and UNDERSTAND the protocol that they plan to use and have VERY GOOD experimental design so that usable material is the result (i.e., labeled appropriately). Users who wish to do amplification of small quantities of RNA should meet with MCF before starting their experiment to make sure their experimental design is appropriate.