2005 Conference Protocols
- Embedding with LRwhite for cross-sections using microtome
Tony Krentz - Biolistic method for transient expression of GFP-fusion protein
Senthil Padmanaban
2004 Conference Protocols
- Metal tolerance assay of yeast on agar plates, or "frogging"
Toshiro Shigaki
- Protocol for analyzing ion sensitivity of Arabidopsis cation transporter mutants
Ning-Hui Cheng
- LiOAc Yeast Transformation
Hirschi Lab - Preparation of MS medium with low K+ or different pH
Heven Sze - Arabidopsis pollen tube germination protocol
Xiyan Li - RNA isolation and RT-PCR from Arabidopsis seedlings
Senthilkumar Padmanaban - Ammonium Protocol
Wen-Ping Chen - Boron Toxicity Test
Wen-Ping Chen - Hypocotyl Growth Assay
Wen-Ping Chen - PEG 6000 Protocol
Wen-Ping Chen - Seed Sterilization Protocol
Wen-Ping Chen - Petri Dish Freezing Assay
Wen-Ping Chen - RT-PCR Analysis
Chris Wilson - Arabidopsis DNA Extraction (CTAB Method)
Chris Wilson
Lab Protocols
- Cre-LoxP for Promoter GUS fusion
- Recommended reaction for
Cre-LoxP
- Univector
Plasmid-Fusion System for Plant Biology
- Comments
on the Cre-lox recombination system
- Construction
of pUNI vector p713-909 for GUS fusion
- p713-909
- Cloning in p713-909
- pHOST=p713-905
- Transcriptional
fusion system for yeast expression
- Troubleshooting:
problems associated with Cre-lox
- References
- 2X LZM Media 12
- Metal Conditions for Yeast
Growth
- DY1457 Yeast expressing 3040, 3040CAX11, or
3040sCAX11 on various metals
- Thirty-Second Arabidopsis Genomic DNA Preparation
for PCR (submitted for publication-- journal article coming soon!)