Recommended reaction for Cre-LoxP

Reaction, typically:

pUNI pHOST 10x Buffer Water Cre 1µl* 1µl* 2µl* 15µl* 1µl* 20µl*

37°C

20 min

Transform in DH5. Select on Amp.

*Qiagen miniprep DNA, approx. 200 ng/µl