The following metal concentrations are good concentrations to see metal sensitivity
in yeast. Photographs of yeast growth of DY1457, 5716, zrc1, cot1, and zrc1 cot1
can be found on page 11/25/02 in Sherry’s Notebook #2. Note that 600µL
of 5M HCl was added to 600mL of YPD to bring the final concentration to 5mM HCl
and pH to around 5.5. This acid addition is critical for good metal solubility
and greatly decreases the amount of metal required to see toxicity.
CaCl2:
200mM & 400mM
NaCl:
500mM & 1M
MnCl2:
2.5mM & 5mM
ZnCl2:
2.5mM & 5mM
CdCl2 :
5µM & 10 µM (note this is micomolar whereas others are millimolar)