2X LZM Media

500 mL:

1.7 g YNB without amino acids and Zn
5 mL 1 M trisodium citrate pH 4.2
1 g –6 amino acid dropout mix
25 µL 1 M MnCl2
250µL 100 mM FeCl2
1 g Potassium Phosphate Monobasic
MQH2O to 500 mL
Autoclave

Before using dilute to 1X and add EDTA to 1 mM, HCl to 5 mM, and glucose to 2%

Reference: Gitan, RS, Luo, H, Rodgers, J, Broderius, M, and Eide, D. (1998). Zinc-induced inactivation of the yeast ZRT1 zinc transporter occurs through endocytosis and vacuolar degradation. Journal of Biological Chemistry. 273 (44): 28617-28624.