| Term 1 |
|
| Ethics - Year 1 |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Aug 30 |
2:30 |
(1L1) Data aquisitioin and record keeping responsibilities - responsibilities for record keeping, challenges in keeping accurate and understandable notebooks, ownership of research materials, reagent/data sharing |
Slaughter |
| 2 |
Tue Sep 06 |
2:30 |
(1L2) Responsible authorship and publication - giving credit when credit is due, when to cite or not, how to use direct quotes, paraphrasing, plagiarism, copyright and copyright permission |
Gilbert |
| 3 |
Wed Sep 14 |
10:00 |
(1C)Case Studies - on record keeping, ownership of research materials, citation and plagiarism |
Faculty |
| 4 |
Tue Sep 20 |
2:30 |
(1L3) The Mentor/Student relationship - Selecting a lab and developing a group of mentors, what to look for in rotations, keeping on track toward your degree |
Sazer |
| Genetics A |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Aug 09 |
1:15 |
Principles, Model Systems and Nomenclature - Mendel's laws. The chromosomal basis of inheritance, definitions of genes, alleles, mutants. Sex linkage. Life cycles and nomenclature of key model systems. Haploid vs. diploid genetics, tetrad analysis in yeast. |
Kelley |
| 2 |
Thu Aug 11 |
1:15 |
Genetic Linkage - Building a genetic map based on recombination frequency. Ordering genes by three factor crosses. Ordering genes by deletion mapping. |
Kelley |
| 3 |
Tue Aug 16 |
1:15 |
Complementation - Complementation tests, allelism. Verification by linkage mapping. Genetic interactions of unlinked loci. Allelic series, penetrance, and expressivity. |
Kelley |
| 4 |
Fri Aug 19 |
1:15 |
Genetic Screens Molecular Basis of the Phenotype - Conception and design of genetic screens. Verification and evaluation of results. Modern genetic philosophy and practice. Classes of mutations. |
Kelley |
| 5 |
Thu Aug 25 |
1:15 |
Bacterial Genetics I - Genetic transmission: haploid and circular genomes, P1-transduction, F-factors, HFRs, conjugation. |
Rosenberg |
| 1 |
Fri Aug 26 |
5:15 |
GA-TA1 - General Genetic Principles |
|
| 6 |
Tue Aug 30 |
1:15 |
Bacterial Genetics II - Phage lambda: lysis versus lysogeny, Campbell model of integration, site specific recombination, establishing repression, host factors, specialized transduction, induction. |
Rosenberg |
| 7 |
Thu Sep 01 |
1:15 |
Bacterial Genetics III - Phage lambda in recombination studies. |
Rosenberg |
| 8 |
Thu Sep 08 |
1:15 |
Bacterial Genetics IV - SOS response. |
Rosenberg |
| 9 |
Tue Sep 13 |
1:15 |
Classes of mutations - Genetics as a study of how proteins interact, fold, and function. Nonsense suppression of null alleles. Partial loss of function alleles. Conditional alleles |
Bates |
| 10 |
Thu Sep 15 |
1:15 |
Delineating a Pathway - How to identify genes that are functionally related to your favorite gene. Suppression analysis. Synthetic lethal mutants |
Bates |
| 2 |
Fri Sep 16 |
5:15 |
GA-TA2 - Phenotype, pathway analysis and genetics screens |
|
| 11 |
Tue Sep 20 |
1:15 |
Enhancer screens and Pathway Analysis - Enhancer screens. Epistasis grouping and epistasis analysis. Establishing the order of gene functions. |
Bates |
| 3 |
Fri Sep 23 |
5:15 |
GA-TA3 - Bacterial Genetics |
|
| |
Wed Sep 28 |
1:15 |
Faculty Review - |
|
| |
Thu Sep 29 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Molecular Methods |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Mon Aug 01 |
1:15 |
Introduction - Objectives of course, integrating methodology into discovery |
Gilbert |
| 2 |
Wed Aug 03 |
1:15 |
Properties of DNA - DNA structure, melting, Tm, annealing, hybridization, gel electrophoresis, blots (Northern, Southern, colony) |
Highlander |
| 3 |
Fri Aug 05 |
1:15 |
DNA Analysis - Probes and labeling, polymerases, kinase and reverse transcriptase, restriction enzymes and mapping, cloning, ligation |
Highlander |
| 4 |
Mon Aug 08 |
1:15 |
DNA manipulation - Sanger DNA sequencing, PCR, quantitative PCR, rtPCR, site directed and random mutagenesis |
Highlander |
| 1 |
Tue Aug 09 |
5:15 |
MM-TA1 - Lectures 1-4 |
|
| 5 |
Wed Aug 10 |
1:15 |
Vectors&Hosts - Plasmids (properties, purification); transformation and selection in E. coli; ColE1-based vectors, TOPO vectors; recombinant cloning vectors. BACs.' M13 (life cycle, vectors, phagemids). Lambda vectors (biology, recombinant construction, in vitro packaging and cosmids). PACs and YACs |
Highlander |
| 6 |
Fri Aug 12 |
1:15 |
Libraries and screening - Construction and handling of libraries with different vectors; cDNA libraries (statistics, subtracted libraries, normalized libraries, reference libraries such as IMAGE); immunological and hybridization screening; genomic libraries (sizes, PCR- and hybridization methods for analyzing) |
Scaduto |
| 7 |
Mon Aug 15 |
1:15 |
Next Generation Sequencing - mapping strategies (FISH mapping, RH mapping), genome sequencing strategies (BAC-by-BAC, library construction, STS or FP, contig assembly, minimum tiling paths, whole genome shotgun), HTP instrumentation (96 capillary machines), data analysis (bsecalling, |
Metzker |
| 2 |
Tue Aug 16 |
5:15 |
MM-TA2 - Lectures 5-7 |
|
| |
Wed Aug 17 |
1:15 |
Midterm Review - |
|
| |
Thu Aug 18 |
1:15 |
Midterm Exam - |
|
| 8 |
Tue Aug 23 |
1:15 |
Sanger Sequencing and Applications - annotation of sequences, EST's, specific organism databases |
Metzker |
| 9 |
Fri Aug 26 |
1:15 |
Protein Expression - Expression in E. coli; transcription and translation signals, production of native proteins,fusion proteins, baculovirus expression systems, in vitro translation |
Gilbert |
| 10 |
Mon Aug 29 |
1:15 |
Protein Purification - Opening the cell, inhibition of proteolysis, precipitants, chromatography (gel filtration, ion exchange, hydrophobic interaction, affinity, HPLC, reversed phase), ultracentrifugation, isoelectric focusing and electrophoresissucrose density gradient,deterg |
Gilbert |
| 11 |
Wed Aug 31 |
1:15 |
Isolation/Refolding Strategies - Inclusion bodies, protein refolding strategies to avoid aggregation, disulfide formation and artifical chaperones |
Gilbert |
| 12 |
Fri Sep 02 |
1:15 |
Flow Cytometry - introduction to the technique of flow cytometry and cell sorting, detecting fluorescent cells through expressed antigens and endogeneous fluorescent moledules |
Sederstron |
| 13 |
Wed Sep 07 |
1:15 |
Interaction cloning - systems, false positives and negatives happen, limitations, combinatorial screens, phage display, two-hybrid screen, lambda gt11 screens |
Pan |
| 3 |
Wed Sep 07 |
5:15 |
MM-TA3 - Lectures 8-11 |
|
| 14 |
Fri Sep 09 |
1:15 |
Protein Localization/Identification - Purification of antigen, polyclonal antibodies, monoclonal antibodies, anti-peptide antibodies, Western blots, GFP fusions, FRAP |
Pan |
| 15 |
Mon Sep 12 |
1:15 |
High throughput methods - protein arrays, interaction networks |
Pan |
| 4 |
Tue Sep 13 |
5:15 |
MM-TA4 - Lectures 12-14 |
|
| 16 |
Wed Sep 14 |
1:15 |
Proteomics - mass spectrometry - mass spectometery (MALDI-TOF, electrospray) protein identification |
Malovannaya |
| 17 |
Fri Sep 16 |
1:15 |
Proteomics - protein identification - identification of protein species in large complexes |
Malovannaya |
| 5 |
Tue Sep 20 |
5:15 |
MM-TA5 - Lectures 15-17 |
|
| |
Mon Sep 26 |
1:15 |
Faculty Review - |
|
| |
Tue Sep 27 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Organization of the Cell |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Mon Aug 01 |
2:30 |
Overview and the Secretory Pathway - Course organization, outline etc. General protein expression and maturation. The secretory pathway and nature of various organelles. |
Sifers |
| 2 |
Wed Aug 03 |
2:30 |
Nuclear Transport - Overall structure of the nucleus and nuclear pore complexes. Transport of proteins and RNAs into and out of the nucleus. Nuclear transport and the control of gene expression |
Sifers |
| 1 |
Thu Aug 04 |
5:15 |
OC-TA1 - Review general cell structures, their function and generation. Concepts of vesicular transport and protein import and export for the organelles |
|
| 3 |
Fri Aug 05 |
2:30 |
Mitochondria, Choloroplasts, Peroxisomes - Structure and biogenesis of mitochondria, chlorplasts, and peroxisomes. Protein import into these organelles |
Sifers |
| 4 |
Wed Aug 10 |
2:30 |
The Endoplasmic Reticulum - quality control checkpoint and mother of the endomembrane system |
Sifers |
| 2 |
Thu Aug 11 |
5:15 |
OC-TA2 - Review of protein trafficking |
|
| 5 |
Fri Aug 12 |
2:30 |
The Golgi Complex - regulated vesicular trafficking in the endomembrane system |
Sifers |
| 6 |
Mon Aug 15 |
2:30 |
Vesicular Transport in Reverse - the plasma membrane-endosome-lysosome connection |
Sifers |
| 7 |
Fri Aug 19 |
2:30 |
Apoptosis - Apoptosis versus necrosis, mechanisms of programmed cell death, regulators of apoptosis. |
Sifers |
| |
Mon Aug 22 |
1:15 |
Midterm Review - |
|
| |
Wed Aug 24 |
1:15 |
Midterm Exam - |
|
| 3 |
Thu Aug 25 |
5:15 |
OC-TA3 - review of apoptosis |
|
| 8 |
Fri Aug 26 |
2:30 |
G Proteins - Signalling mechanisms, ligand recognition and conformation changes by receptors, GDP release kinetics, G protein structure and function, effectors, signal processing, inactivation and desensitization, receptor kinases and arrestins, putative roles for PKC |
Wensel |
| 9 |
Mon Aug 29 |
2:30 |
Growth Factors/Tyrosine Kinase Signaling - Ligands and signals, structure of families of membrane receptors with intrinsic tyrosine kinase activity and introduction to cascades activated by binding to phosphotyrosines, SH2 and SH3 domains, tyrosine kinases associated with membrane bound receptors |
Wensel |
| 10 |
Wed Aug 31 |
2:30 |
Calcium and phospholipase signaling - Calcium and its protein complexes Phosphoinositides, phospholipase C, protein kinase C, IP3 receptors, proteins regulated by Ca, calmodulin, Ca-regulated channels, annexins, overview of methods for monitoring intracellular Ca and phosphoplipase activity |
Wensel |
| 4 |
Thu Sep 01 |
5:15 |
OC-TA4 - Review of G-protein signaling and tyrosine kinase signaling |
|
| 11 |
Fri Sep 02 |
2:30 |
Cytoskeleton I - Actin, microtubules, intermediate filaments, junctional complexes and cellular architecture, cell motility. |
He |
| 12 |
Wed Sep 07 |
2:30 |
Cytoskeleton II - Actin, microtubules, intermediate filaments, junctional complexes and cellular architecture, cell motility. |
He |
| 5 |
Thu Sep 08 |
5:15 |
OC-TA5 - Review of cytoskeleton and calcium signaling |
|
| 13 |
Fri Sep 09 |
2:30 |
Energy Transduction and Bioenergetics - Membrane permeability, mechanisms of transport, bio-energetics, mitochondrial and chloroplast function, chemiosmosis |
Pedersen |
| 14 |
Wed Sep 14 |
2:30 |
Transport Mechanisms - Active and passive transport, synporters, antiporters, ATPases |
Pedersen |
| 6 |
Thu Sep 15 |
5:15 |
OC-TA6 - review of bioenergetics, transporters, ion channels |
|
| 15 |
Fri Sep 16 |
2:30 |
Ion channels - Nernst potential, electrical properties of excitable membranes, action potential |
Pedersen |
| |
Wed Sep 21 |
1:15 |
Faculty Review - |
|
| |
Thu Sep 22 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Science as a Profession |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Aug 02 |
2:30 |
Organization of the literature - Organization and purpose of a paper, how to read the current literature, what is contained in the various sections of a paper, computer searching vs browsing, parts of a scientific paper and what you can learn from each |
Slaughter |
| 2 |
Tue Aug 09 |
2:30 |
Setting goals for your scientific development and coping with challenges - Career paths for the professional scientist, career decisions (how and when to make them), what to do in your first year to ensure your success, developing curiosity, coursework(what to expect in gradschool), lifelong learning, the scientific literature, finding a lab/rotation, selecting a mentor, where to get help and information, thinking now about the next step |
Basinger |
| 3 |
Tue Aug 16 |
2:30 |
The Scientific Method - thinking and talking like a scientist - The scientific method (hypothesis vs results-driven science), elements of experimental design (developing models/hypotheses), designing experimental tests of your hypotheses, fishing expeditions, controls , replication of experiments and data selection - ethical considerations, Interpreting your results |
Gilbert |
| 4 |
Tue Aug 23 |
2:30 |
The funding structure of science and public policy - Government organizations, private funding sources, applying for fellowships, structure of a grant, grant review system, science advocacy |
Brinkley |
| Term 2 |
| Cancer |
| No |
Date |
Time |
Lecture |
Lecturer |
| |
Thu Oct 20 |
2:30 |
Graduate Student Symposium - No Class - |
|
| 1 |
Fri Nov 18 |
1:15 |
Introduction to Cancer Course - Oncogenes - leukemia/lymphoma |
Plon |
| 2 |
Mon Nov 21 |
1:15 |
Amimal Models in Cancer Research - lung cancer |
Demayo |
| 3 |
Wed Nov 23 |
1:15 |
Cancer as a Multi-step Process - tumor suppressor genes, colon cancer |
Plon |
| |
Thu Nov 24 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| |
Fri Nov 25 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| 4 |
Mon Nov 28 |
1:15 |
Use of familial Cancers to Define Molecular Events - breast cancer |
Plon |
| 5 |
Wed Nov 30 |
1:15 |
Metastasis - |
Demayo |
| |
Tue Dec 06 |
1:15 |
Faculty Review - |
|
| |
Wed Dec 07 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Cell Division |
| No |
Date |
Time |
Lecture |
Lecturer |
| 2 |
Mon Oct 10 |
1:15 |
Principles of cell cycle regulation - CDKs, cyclins, CKIs, and proteolysis |
Zhang |
| 1 |
Mon Oct 10 |
1:15 |
Course Introduction/Cell Cycle Overview - Discovery of cell cycle regulation--Cell cycle stages, mitosis and meiosis, discovery of CDK/cyclin/MPF |
He |
| 1 |
Tue Oct 11 |
5:15 |
CD-TA1 - Review of cell cycle basics |
He |
| 3 |
Fri Oct 14 |
1:15 |
Principles of cell cycle regulation - cell cycle transitions, G1/S and G2M |
Zhang |
| 4 |
Mon Oct 17 |
1:15 |
S phase - Initiation and control of DNA replication at origins of replications |
Wang |
| 2 |
Tue Oct 18 |
5:15 |
CD-TA2 - Review of Mitosis |
He |
| 5 |
Wed Oct 19 |
1:15 |
DNA Damage - DNA damage and repair |
Wilson |
| |
Thu Oct 20 |
2:30 |
Graduate Student Symposium - No Class - |
|
| 6 |
Mon Oct 24 |
1:15 |
DNA Repair - Repair of double strand breaks by homologous and non-homologous recombination |
Wilson |
| 3 |
Tue Oct 25 |
5:15 |
CD-TA3 - Review of DNA replication and recombination |
Wilson/Wang |
| 7 |
Wed Oct 26 |
1:15 |
Cell Cycle Checkpoints - Introduction to cell cycle checkpoints, the DNA damage checkpoint |
Zhang |
| |
Thu Oct 27 |
1:15 |
Faculty Review Midterm - Lectures 1-6 |
|
| |
Fri Oct 28 |
1:15 |
Exam I - Lectures 1-6 |
|
| 8 |
Mon Oct 31 |
1:15 |
M phase chromosomes - chromosomes (centromeres, telomeres), chromosome pairing and chromosomes (centromeres, telomeres), chromosome pairing and cohesion, chromosome condensation |
He |
| 9 |
Wed Nov 02 |
1:15 |
M phase spindle - mechanisms of spindle formation and function, kinetochore/spindle attachment |
He |
| 4 |
Wed Nov 02 |
5:15 |
CD-TA4 - Review of CDK/cyclin cell cycle transitions |
Zhang |
| 10 |
Fri Nov 04 |
1:15 |
Spindle Assembly Checkpoint - the spindle assembly checkpoint. Exit from mitosis, coordination of exit from mitosis with cytokinesis |
He |
| 11 |
Wed Nov 09 |
1:15 |
Cytokinesis - cytokinesis mechanisms |
Sokac |
| 5 |
Wed Nov 09 |
5:15 |
CD-TA5 - Review of DNA damage and spindle Assembly checkpoints, mitotic exit, cytokinesis |
Zhang/Sokac |
| 12 |
Fri Nov 11 |
1:15 |
Growth regulation - The cell cycle in growth regulation, development and cancer |
Westbrook |
| |
Tue Nov 15 |
1:15 |
Faculty Review - |
|
| |
Wed Nov 16 |
1:15 |
Exam II - Lectures 7-12 |
|
| |
Thu Nov 24 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| |
Fri Nov 25 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| Development |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Mon Oct 17 |
2:30 |
Core Concepts in Developmental Biology - |
Groves |
| 2 |
Wed Oct 19 |
2:30 |
Setting up the vertebrate body plan I - Axis determination and gastrulation |
Groves |
| |
Thu Oct 20 |
2:30 |
Graduate Student Symposium - No Class - |
|
| 3 |
Fri Oct 21 |
2:30 |
Setting up the vertebrate body plan II - Mammalian development and embryonic stem cells |
Groves |
| 4 |
Wed Oct 26 |
2:30 |
Setting up the vertebrate body plan III - Patterning the embryo from head to tail |
Groves |
| 1 |
Fri Oct 28 |
5:15 |
TA-DE1 - |
|
| 5 |
Mon Oct 31 |
2:30 |
ES cells, pluripotency and reprogramming/iPS - |
Zwaka |
| 6 |
Wed Nov 02 |
2:30 |
Stem cells in the adult - |
Nguyen |
| 2 |
Thu Nov 03 |
5:15 |
TA-DE2 - |
|
| |
Fri Nov 04 |
2:30 |
Mid-Term Faculty Review - |
|
| |
Mon Nov 07 |
1:15 |
Mid-Term Exam - |
|
| 7 |
Wed Nov 09 |
2:30 |
Vertebrate Neural Development I - Developmental anatomy of the nervous system. Molecular aspects of neural induction, formation of the neural plate and neurogenesis |
Deneen |
| 8 |
Fri Nov 11 |
2:30 |
Vertebrate Neural Development II - Molecular mechanisms of dorsal-ventral and anterior-posterior patterning. Combinatorial and antagonistic signaling involved in dorsoventral patterning of the neural tube |
Deneen |
| 2 |
Fri Nov 11 |
5:15 |
TA-DE2 - |
|
| 9 |
Mon Nov 14 |
2:30 |
Limb Development - Axis specification, outgrowth and patterning |
Johnson |
| 3 |
Wed Nov 16 |
5:15 |
TA-DE3 - |
|
| 10 |
Fri Nov 18 |
2:30 |
Introduction to Drosophila embryogenesis - Overview of Drosophila oogenesis and embryogenesis |
Jafar-Nejad |
| 11 |
Mon Nov 21 |
2:30 |
Embryonic patterning in Drosophila - I - Maternal-effect genes and the establishment of the anteroposterior axes |
Jafar-Nejad |
| 3 |
Tue Nov 22 |
5:15 |
TA-DE3 - |
|
| 12 |
Wed Nov 23 |
2:30 |
Embryonic patterning in Drosophilia II - Maternal-effect genes and the establishment of the dorsoventral axes |
Jafar-Nejad |
| |
Thu Nov 24 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| |
Fri Nov 25 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| 4 |
Tue Nov 29 |
5:15 |
TA-DE4 - |
|
| |
Thu Dec 01 |
1:15 |
Faculty Review - |
|
| |
Fri Dec 02 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Ethics - Year 2 |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Oct 11 |
2:30 |
(2L1) Research misconduct - (NIH b3) Safe Practices in the Laboratory - Definitions - Falsification, fabrication, plagiarism - whistleblowers, allegations, investigations, penalties. College policy and mechanisms for handling misconduct allegations, federal policies and procedures. (NIH b3) Safe Practices in the Laboratory - refresher of material covered at orientation regarding laboratory safety |
Gilbert |
| 2 |
Tue Oct 25 |
2:30 |
(2L2) Ethics of Experiments with Animals - when can animals be used ethically in research, avoiding unnecessary pain/suffering and euthanasia, appropriate selection of numbers/types of animals in research, animal use approval |
Buckmaster |
| 3 |
Wed Nov 09 |
10:00 |
(2C) Case Studies - on scientific misconduct and experiments with animals (small group discussion format with faculty facilitator) |
Faculty |
| 4 |
Tue Nov 22 |
2:30 |
(2L3) Mentorship - Meeting with 2nd year students and their mentors, matching expectations between mentor student, developing communication channels, picking an appropriate thesis topic, the thesis committee as a resource |
Gilbert |
| Genetics B |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Oct 11 |
1:15 |
Introduction - Comparisons of classical genetics, reverse genetics, and genomics. Strengths and weaknesses of model systems. Nomenclature. |
Zhou |
| 2 |
Wed Oct 12 |
2:30 |
Yeast I - Basic molecular manipulations in reverse genetics: cloning by complementation, plasmid gap repair, gene disruption/replacement, plasmid shuffle |
Ira |
| 3 |
Tue Oct 18 |
1:15 |
Yeast II - Genome analysis and functional genomics. The impact of the yeast genome project. Systematic manipulation of entire gene sets to monitor mRNA expression and protein localization in vivo. |
Ira |
| 1 |
Wed Oct 19 |
5:15 |
GB-TA1 - |
|
| |
Thu Oct 20 |
2:30 |
Graduate Student Symposium - No Class - |
|
| 4 |
Fri Oct 21 |
1:15 |
C. elegans I- - Advantages and special considerations of hermaphrodite genetics. Significance of having the detailed fate map of the organism. Developmental genetics at the single cell level. |
Zhou |
| 5 |
Tue Oct 25 |
1:15 |
C. elegans II - C. elegans transformation. Positional cloning and complementation. Impact of the C. elegans genome project. Reverse genetics using RNA interference. |
Zhou |
| 6 |
Tue Nov 01 |
1:15 |
Drosophila I - Introduction. Nomenclature and markers; chromosome cytology (polytene chromosomes) and chromosome rearrangements; using balancer chromosomes to follow segregation and to isolate mutations; P-elements and transformation; site-specific integration using the PhiC31 system. |
Mardon |
| 2 |
Tue Nov 01 |
5:15 |
GB-TA2 - |
|
| 7 |
Thu Nov 03 |
1:15 |
Drosophila II - Genetic screens. Mutagens: ems, x-rays and P-elements; classic genetic screens to identify mutations in Drosophila: F1, F2, F3 screens; modifier (suppressor and enhancer) screens; isolating mutations, mapping lethal mutations; deficiency mapping; high-throughput sequencing. |
Mardon |
| 8 |
Tue Nov 08 |
1:15 |
Drosophila III - Gene function. Mosaic analysis; autonomy versus non-autonomy; mitotic recombination using the FLP/FRT system, methods of marking clones; ectopic expression using heat shock, GAL4-UAS, and flp-out; MARCM analysis. |
Mardon |
| 9 |
Thu Nov 10 |
1:15 |
Mouse Genetics I - Inbred mice. Microsatellite markers. Mapping genes in mice using crosses. |
Matzuk |
| 3 |
Thu Nov 10 |
5:15 |
GB-TA3 - |
|
| 10 |
Mon Nov 14 |
1:15 |
Mouse Genetics II - Insertion of DNA and retrotransposons. Knock-outs and ins. Conditional knock-outs. |
Matzuk |
| 11 |
Thu Nov 17 |
1:15 |
Human Genetics I - Disease loci and pedigrees. |
Lupski |
| 4 |
Fri Nov 18 |
5:15 |
GB-TA4 - |
|
| |
Thu Nov 24 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| |
Fri Nov 25 |
1:15 |
Thanksgiving Holiday - No Class - |
|
| 12 |
Mon Nov 28 |
2:30 |
Human Genetics II - Mapping with DNA markers. Sources of polymorphisms. Simple vs. complex traits. Genome scans for quantitative trait loci. |
Lupski |
| 13 |
Tue Nov 29 |
1:15 |
Human Genetics III - Effect of imprinting on pedigrees. Models for specific mechanisms of imprinting. Model for evolutionary significance. |
Lupski |
| 5 |
Wed Nov 30 |
5:15 |
GB-TA5 - |
|
| |
Thu Dec 08 |
1:15 |
Faculty Review - |
|
| |
Fri Dec 09 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Method and Logic in Molecular Biology |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Oct 11 |
10:00 |
Method and Logic Meeting - |
Catanese |
| 1 |
Thu Oct 13 |
10:00 |
Method and Logic Meeting - |
Catanese |
| 3 |
Tue Oct 18 |
10:00 |
Method and Logic Meeting - |
Moore |
| 4 |
Thu Oct 20 |
10:00 |
Method and Logic Meeting - |
Bertuch |
| 5 |
Tue Oct 25 |
10:00 |
Method and Logic Meeting - |
Suter |
| 6 |
Thu Oct 27 |
10:00 |
Method and Logic Meeting - |
Chen |
| 7 |
Tue Nov 01 |
10:00 |
Method and Logic Meeting - |
Deneen |
| 8 |
Thu Nov 03 |
10:00 |
Method and Logic Meeting - |
Frankfort |
| 9 |
Tue Nov 08 |
10:00 |
Method and Logic Meeting - |
Zechiedrich |
| 10 |
Thu Nov 10 |
10:00 |
Method and Logic Meeting - |
Mardon |
| 12 |
Thu Nov 17 |
10:00 |
Method and Logic Meeting - |
Shaulsky |
| 12 |
Thu Nov 17 |
10:00 |
Method and Logic Meeting - |
Wang |
| 13 |
Tue Nov 22 |
10:00 |
Method and Logic Meeting - |
He |
| 14 |
Tue Nov 29 |
10:00 |
Method and Logic Meeting - |
Scott |
| 15 |
Thu Dec 01 |
10:00 |
Method and Logic Meeting - |
Groves |
| Term 3 |
| Gene Regulation |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Wed Jan 04 |
1:15 |
Introduction to Gene Regulation - Sites of Gene Regulation: transcription initiation, termination, alternative RNA splicing, 3' end processing, nuclear export, cytoplasmic mRNA stability, translational regulation, protein degradation. Analysis of gene expression. |
Cooper |
| 2 |
Fri Jan 06 |
1:15 |
RNA Polymerase - the bacterial enzyme summarizing the kinetics and thermodynamics of the protein and its interactions with DNA, subunit function of the bacterial enzyme compared with the subunit structure of the eukaryotic enzyme. Regulatory functions of the polymerase. |
Herman |
| 3 |
Mon Jan 09 |
1:15 |
Control of transcription in bacteria - degradation and sequestration of initiation factors, riboswitches, noise in transcription and logic of transcriptional circuits. |
Herman |
| 4 |
Wed Jan 11 |
1:15 |
Eukaryotic RNA polymerase and transcriptional initiation - Structures and activities of the 3 eukaryotic RNA polymerases. Pathways for transcription initiation by RNA pol II holoenzyme plus general transcription factors. |
Moore |
| 1 |
Wed Jan 11 |
5:15 |
GRTA1 - Overview of regulation of transcription and translation including review of overall process of transcription and translation, introduce reporter genes (rationale) |
|
| 5 |
Fri Jan 13 |
1:15 |
Modes of DNA binding and basic transcription factors - Structures of DNA binding domains and the molecular basis for DNA sequence recognition. How do transcription factors find their sites? Genome wide networks of transcriptional regulation. |
Moore |
| |
Mon Jan 16 |
1:15 |
Martin Luther King Day Holiday - No Class - |
|
| 6 |
Tue Jan 17 |
1:15 |
Coactivators and corepressors - Identification of transcriptional coregulators and evolution of understanding of transcriptional activation. Enymatic activities of coactivators and corepressors. Post-transcriptional modulation of coregulator function. Why are there so many coregulators? |
Lonard |
| 7 |
Wed Jan 18 |
1:15 |
Chromatin 1 - Local - Histones, nucleosomes, covalent modification of histones, DNA accessibility, insulators, locus control regions, polytene/lapbrush chromosomes. |
Lonard |
| 8 |
Fri Jan 20 |
1:15 |
Chromatin 2 - Global - Chromatin subdomains, gene silencing, position effect variegation, epigenetic inheritance, X-inactivation, gene imprinting. |
Lonard |
| 9 |
Tue Jan 24 |
1:15 |
Chromatin - Relationship between DNA sequence genes, transcription and Chromatin - Emerging pictures of genes and chromosomes. Enabling technologies for global chromatin and transcriptome analysis, evolving understanding of gene structure, promoters, enhancers, relationship between primary DNA sequence, chromatin and transcription. |
Lonard |
| 2 |
Wed Jan 25 |
5:15 |
GR TA2 - Review of assembly of transcription complex including order and identity of factor loading Identify major transcriptional activator families and mechanisms |
|
| 10 |
Fri Jan 27 |
1:15 |
Regulation of RNA Polymerase II Elongation - Transcriptional elongation. Negative factors that limit elongation (NELF, DSIF); Positive factor that activates elongation (P-TEFb); HIV system (Tat, TAR RNA, P-TEFb); Drosophila heat shock genes and elongation; Current questions in this area |
Rice |
| 11 |
Mon Jan 30 |
1:15 |
Nuclear export of RNA - Nuclear pores - structure and dynamics; Retroviral systems: Retroviral life cycle, HIV system (Rev, RRE RNA, CRM1, RAN/GTP), Mason-Pfizer Monkey Virus (MMPV; CTE, TAP); Experimental methods to study RNA export; Export pathways of different classes of cellular RNAs; Current questions in this area. |
Rice |
| |
Tue Jan 31 |
2:30 |
Midterm Faculty Review - |
|
| |
Wed Feb 01 |
1:15 |
Midterm Exam - |
|
| 12 |
Thu Feb 02 |
1:15 |
Splicing of Pre-mRNA (I) - Overview of RNA chemistry (basis for lability and reactivity), overview of RNA structure, comparisons of intron/exon architecture, Autocatalytic RNA: The spliceosome; U12-dependent introns, domain structure of RNA binding proteins. |
Cooper |
| 13 |
Fri Feb 03 |
1:15 |
Splicing of Pre-mRNA (II) - How the spliceosome finds vertebrate exons, intron definition vs exon definition, splicing enhancers and silencers, SR proteins, Exon junction complex (EJC), Trans-splicing. |
Cooper |
| 14 |
Mon Feb 06 |
1:15 |
3' end formation / Polyadenylation - 3' end formation of pol II genes, transcription termination; cotranscriptional 3' end formation; relationship between 3' end formation and splicing, histone 3' end formation. |
Cooper |
| 15 |
Wed Feb 08 |
1:15 |
Alternative splicing - Drosophila paradigms, yeast and vertebrate systems; splicing microarrays; effects on coding potential; regulatory factors and mechanisms of regulation; signaling pathways; splicing and human disease (direct cause and as genetic modifier) |
Cooper |
| 3 |
Wed Feb 08 |
5:15 |
GR TA3 - Splicing and poyadenylation general overview; define mechanisms for alternative splice-site selection, intron vs exon selection |
|
| 16 |
Thu Feb 09 |
1:15 |
RNA (and DNA) Editing - RNA editing, Insertional/deletion editing (Mitochondrial mRNA editing, contrast with trans-esterifiction, editsome complex, spliceosome), tRNA editing and codon recognition, ADAR activity and function, interplay with the microRNA pathway, AMPA receptor, cytosine deaminases. |
Neilson |
| 17 |
Mon Feb 13 |
1:15 |
Noncoding RNA Function - Biogenesis and function of microRNAs. MicroRNA target prediction. RNAi. Modes of effecting RNAi in eukaryotic systems and forward genetic screens with shRNA libraries. LincRNAs and chromatin. |
Neilson |
| 18 |
Wed Feb 15 |
1:15 |
Posttranscriptional mRNA Regulation - The impact of turnover on steady state RNA levels. e.g. histone gene expression during the cell cycle. Modes and mechanisms of RNA decay. Poly A shortening and 5' -> 3' degradation, P-bodies and mRNA decay. Non-polyA shortening mechanisms. Translation Coupled mRNA Degradation. Nonsense-mediated mRNA decay. Role of 3'UTR sequences in mRNA localization. |
Neilson |
| 19 |
Fri Feb 17 |
1:15 |
Translational Regulation I - Review of protein synthesis and initiation steps, Cap-dependent and cap independent translation. 5'-3' interactions and recycling. Global regulation mechanisms; eIF2 kinase mechanisms, eIF4E inhibitors, viral mechanisms, mTOR, TOP mRNAs. |
Lloyd |
| |
Mon Feb 20 |
1:15 |
President's Day Holiday - No Class - |
|
| 20 |
Tue Feb 21 |
1:15 |
Translational Regulation II Mechanisms - mRNA-specific regulation; scanning blockers, (uORFs, IRE, autoregulatory systems), cellular IRESs, shunt mechanisms, 3' UTR-based regulation (CPEB in oocytes and neurons) - stress mechanisms (stress granules and P bodies), Frameshifting. |
Lloyd |
| 21 |
Wed Feb 22 |
1:15 |
Regulation of Protein Turnover - Ubiquitin-proteosome mediated degradation. Importance of regulation, protein motifs, PEST sequences, etc. |
Lloyd |
| 4 |
Tue Feb 28 |
5:15 |
GR-TA4 - RNA self-splicing overview, concept of regulating synthesis and degradation of mRNA, review of ribosome assembly and initiation and termination of translation |
|
| |
Thu Mar 01 |
1:15 |
Faculty Review - |
|
| |
Fri Mar 02 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Immunology |
| No |
Date |
Time |
Lecture |
Lecturer |
| |
Mon Jan 16 |
1:15 |
Martin Luther King Day Holiday - No Class - |
|
| 1 |
Thu Feb 02 |
2:30 |
Principles of specific immunity - innate vs. specific immunity, cells of the immune system, clonal selection principle, strategies for self-nonself discrimination |
Decker |
| 2 |
Tue Feb 07 |
2:30 |
Antibody structure, antigen receptor diversity and B-cell development - Antigens for B cells, Antibody receptor structure and genetics, Generation of diversity, Gene rearrangements and class switching |
Levitt |
| 3 |
Thu Feb 09 |
2:30 |
B-cell activation, MHC and antigen presentation - B cell development and selection. Self vs. nonself selection, Tolerance, T cell antigen recognition, MHC structure and function. MHC antigen presentation and processing. |
Levitt |
| 1 |
Tue Feb 14 |
5:15 |
IM TA Review 1 - Overview of response to pathogen: Anatomy and function of organs of lymphatic system: general overview of B and T cell development and function |
|
| 4 |
Thu Feb 16 |
2:30 |
Role and development of T lymphocytes - Types of T cells, T cell selection and self tolerance. T cell activation, signal transduction, convergence and integration of signal transduction pathways, anergy, apoptosis |
Decker |
| |
Mon Feb 20 |
1:15 |
President's Day Holiday - No Class - |
|
| 5 |
Tue Feb 21 |
2:30 |
T cell development and signaling: consequences for transplantation and tumors - T cell effector functions, transplantation, autoimmunity, tumor immunology |
Decker |
| 2 |
Tue Feb 21 |
5:15 |
IM TA Review 2 - MHC I and MHC II - functions, derivation; B cells - antibody structure - subtypes, receptors and surface molecules, VDJ recombination; T cells - receptor and surface molecules, maturation, subtypes/function; T and B cell interactions |
|
| |
Thu Feb 23 |
1:15 |
Faculty Review - |
|
| |
Mon Feb 27 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Macromolecules: Structure and Interactions |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Jan 03 |
2:30 |
Protein Structure - Protein structure elements: from building blocks to quaternary structure; protein topology; "Fold follows function"; protein taxonomy. |
Tsai |
| 2 |
Wed Jan 04 |
2:30 |
Protein Folding - Protein folding in vitro (Anfinsen theorem); energy landscapes; molecular chaperones and protein folding in vivo; protein misfolding diseases. |
Tsai |
| 3 |
Fri Jan 06 |
2:30 |
DNA Structure - DNA Structure: A-, B- and Z- DNA structure; building blocks; DNA topology; from DNA to RNA |
Tsai |
| 4 |
Mon Jan 09 |
2:30 |
Lipid/Membrane Structure - Lipid/Membrane Structure: Lipid classification; micelles and bilayers; membrane structures; membrane proteins. |
Tsai |
| 5 |
Wed Jan 11 |
2:30 |
Protein - post-translational modifications - Common reversible and irreversible post-translational modifications: regulation and roles in protein function |
Weigel |
| 1 |
Thu Jan 12 |
5:15 |
MSI-TA Review 1 - |
|
| 6 |
Fri Jan 13 |
2:30 |
X-ray/Cryo EM - Scattering, (diffraction)-based technique, diffraction theory, crystallization, symmetry. EM single particle methods, averaging |
Schmid |
| 7 |
Wed Jan 18 |
2:30 |
NMR - Basic theory and instrumentation, NOESY, COSY methods, distance geometry, multi-dimensional NMR, sample requirements. |
Schmid |
| 2 |
Thu Jan 19 |
5:15 |
MSI-TA Review 2 - |
|
| 8 |
Fri Jan 20 |
2:30 |
Fluorescence/optical spectroscopy - Principles of photon absorption and emission, fluorescence energy transfer, anisotropy, lifetime instrumentation, optics. |
Schmid |
| 9 |
Mon Jan 23 |
1:15 |
Ligand Binding - General Principles of ligand binding formalism, mass action, and basic energetics. Techniques for doing binding analysis. |
Pedersen |
| |
Mon Jan 23 |
2:30 |
Midterm Faculty Review - |
|
| |
Wed Jan 25 |
1:15 |
Midterm Exam - |
|
| 10 |
Fri Jan 27 |
2:30 |
Forces in Solution/Thermodynamics - Basic forces in solution. What holds structures together? Thermodynamics of binding and how the relate to basic forces. |
Pedersen |
| 3 |
Fri Jan 27 |
5:15 |
MSI-TA Review 3 - |
|
| 11 |
Mon Jan 30 |
2:30 |
Allostery 1 - Protein dynamics and structural allostery and how it mediates and regulates protein function. |
Pedersen |
| 12 |
Tue Jan 31 |
1:15 |
Allostery and Cooperativity - How protein structural changes mediate cooperative effects in enzymes and binding proteins |
Pedersen |
| 4 |
Thu Feb 02 |
5:15 |
MSI-TA Review 4 - |
|
| 13 |
Fri Feb 03 |
2:30 |
Binding Kinetics - Basic kinetics of unimolecular and bimolecular reactions. |
Pedersen |
| 14 |
Mon Feb 06 |
2:30 |
Enzyme Kinetics/Catalysis - Michaelis-Menten kinetics, Inhibition, kcat and kcat/Km, allosteric enzymes, inhibition, practical features of an assay |
Petrosino |
| 15 |
Wed Feb 08 |
2:30 |
Mechanisms of Catalysis - Acid base catalysis, covalent catalysis, stabilization of transition states, transition state analogs, coenzymes, suicide inhibitors |
Petrosino |
| 5 |
Thu Feb 09 |
5:15 |
MSI-TA Review 5 - |
|
| 16 |
Mon Feb 13 |
2:30 |
Single Molecule methods - Macromolecular motors, single molecule kinetics, examples |
Petrosino |
| 17 |
Wed Feb 15 |
2:30 |
Protein - small molecule interactions - Molecular basis of specificity, protein ligand interactions - structural basis of H-bonding, salt bridges and other interactions |
Prasad |
| 18 |
Fri Feb 17 |
2:30 |
Protein - Protein interactions - Protein-protein interactions. Structural motifs that serve as protein-protein interaction domains, structural basis of surface complimentarity, engineering tight binding. |
Prasad |
| 19 |
Wed Feb 22 |
2:30 |
Protein/DNA interactions - Protein-DNA interactions. DNA binding motifs, structural basis of base recognition, backbone interactions |
Prasad |
| 20 |
Fri Feb 24 |
2:30 |
Systems Biology - Some principles and examples from systems biology approaches to understanding signaling in regulatory networks |
Pedersen |
| |
Tue Feb 28 |
1:15 |
Faculty Review - |
|
| |
Wed Feb 29 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Neuroscience |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Thu Jan 05 |
2:30 |
Cellular and Structural Neuroanatomy - Major subdivions of the mammalian brain, their general organization and the specialized cells required for function. |
Jankowsky |
| 2 |
Tue Jan 10 |
2:30 |
Ion Channels and Action Potentials - Structure and function of membrane proteins responsible for establishing ionic gradients in neurons and how they use ionic imbalances to transmit electrical information. |
Jankowsky |
| 3 |
Thu Jan 12 |
2:30 |
Synapses and Postsynpatic Receptors - Specialized structures and proteins that allow electrical signals to be converted to chemical messengers and then back again |
Jankowsky |
| 4 |
Tue Jan 17 |
2:30 |
Learning and Memory - Molecular and cellular requirements for storing new information and recalling it later |
Jankowsky |
| 5 |
Thu Jan 19 |
2:30 |
Neurobiology of Disease - What happens when things go wrong in the brain. |
Jankowsky |
| |
Tue Jan 24 |
2:30 |
Faculty Review - |
|
| |
Thu Jan 26 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| Term 4 |
| Basic Biostatistics |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Mar 13 |
1:15 |
Introduction to statistics and why they should be important to you - |
Paylor |
| 2 |
Thu Mar 15 |
1:15 |
Data & measurements scales - |
Paylor |
| 3 |
Tue Mar 20 |
1:15 |
Descriptive statistics - |
Paylor |
| 4 |
Thu Mar 22 |
1:15 |
Distributions - |
Paylor |
| 5 |
Tue Mar 27 |
1:15 |
Z-scores - |
Paylor |
| 6 |
Thu Mar 29 |
1:15 |
Hypothesis testing & experimental design - |
Paylor |
| 7 |
Tue Apr 03 |
1:15 |
Analyses of two independent cases - |
Paylor |
| 8 |
Thu Apr 05 |
1:15 |
Analyses of multiple independent cases - |
Paylor |
| 9 |
Tue Apr 10 |
1:15 |
Analyses of within subject designs, related cases - |
Paylor |
| 10 |
Tue Apr 17 |
1:15 |
Analyses of interactions - |
Paylor |
| 11 |
Thu Apr 19 |
1:15 |
Follow-up analyses - |
Paylor |
| 12 |
Tue Apr 24 |
1:15 |
Non-parametric analyses - |
Paylor |
| 13 |
Thu Apr 26 |
1:15 |
Correlations/Data presentations - |
Paylor |
| 14 |
Tue May 01 |
1:15 |
Power analyses - |
Paylor |
| 15 |
Thu May 03 |
1:15 |
Student & Instructor Evaluation for Future Course Improvement - |
Paylor |
| |
Fri May 11 |
1:15 |
Final Exam - 1:15-3:45 Due - |
|
| Research Design |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Tue Mar 13 |
1:00 |
Research Design Introduction - Introduction to the design of research projects |
Gilbert |
| 2 |
Tue Mar 20 |
1:00 |
Research Design Workgroup 2 - Introduction to the design of research projects |
Schmid |
| 3 |
Tue Mar 27 |
1:00 |
Research Design Workgroup 3 - Introduction to the design of research projects |
Gilbert |
| 4 |
Tue Apr 03 |
1:00 |
Research Design Workgroup 6 - Introduction to the design of research projects |
Prasad |
| 5 |
Tue Apr 10 |
1:00 |
Research Design Workgroup 3 - |
Staff |
| 6 |
Tue Apr 17 |
1:00 |
Research Design Workgroup 4 - |
Staff |
| 7 |
Tue Apr 24 |
1:00 |
Research Design Workgroup 7 - |
Staff |
| 8 |
Tue May 01 |
1:00 |
Research Design Workgroup 8 - |
Staff |
| 9 |
Thu May 10 |
1:00 |
Research Design Symposium - |
Staff |
| Structure of Macromolecules |
| No |
Date |
Time |
Lecture |
Lecturer |
| 1 |
Mon Mar 12 |
1:15 |
Protein Taxonomy - Discussion of protein motifs, domains and general structural features. Approaches to function from structure. |
Tsai |
| 2 |
Wed Mar 14 |
1:15 |
Protein Folding - Mechanisms for attaining the correct 3D structure of proteins, experimental approaches to observing protein folding, catalysis of protein folding |
Gilbert |
| 3 |
Fri Mar 16 |
1:15 |
NMR of proteins - Basic theory and instrumentation, NOSEY, COSY methods, distance geometry, multi-diminsional NMR, sample requirements |
Gilbert |
| 4 |
Mon Mar 19 |
1:15 |
Hydrodynamic and Spectroscopic methods - methods for estimating size, shape compactness and oligomeric state of proteins (and interactions among them)--analytical ultracentrifugation, native gels, size exclusions chromatography, dynamical light scattering, small angle x-ray scattering CD, FTIR, proton exchange |
Schmid |
| 5 |
Wed Mar 21 |
1:15 |
Sequence to Structure - primary structure analysis, domain classifications, prediction of secondary and tertiary structure |
Lichtarge |
| 6 |
Fri Mar 23 |
1:15 |
Sequence and structure to function - structural genomics and functional site analysis |
Lichtarge |
| 7 |
Mon Mar 26 |
1:15 |
DNA Structure and topology I - Structure of B, Z and A DNA, topology, structural effects on electrophoretic properties of DNA, R-loops, D-loops, Literature project on DNA structure/function |
Zechiedrich |
| 8 |
Wed Mar 28 |
1:15 |
DNA Structure and topology II - Methods of analyzing DNA structure, future goals in field of DNA structure/topology, literature project |
Zechiedrich |
| 9 |
Fri Mar 30 |
1:15 |
Xray crystallography - a scattering (diffraction)-based structural technique. Crystallization and synmetry. Theory of diffraction |
Schmid |
| 10 |
Mon Apr 02 |
1:15 |
Convolution - The diffraction pattern as a Fourier Transform. Some simple examples. |
Schmid |
| 11 |
Wed Apr 04 |
1:15 |
The Phase Problem - solving the phase problem, one way or another. EM techniques. |
Schmid |
| |
Fri Apr 06 |
1:15 |
Good Friday Holiday - No Class - |
|
| 13 |
Mon Apr 09 |
1:15 |
Fluorescence Methods for Structure analysis - principles of photon absorption and emission, fluorescence energy transfer, anisotropy, lifetime, instrumentation, optics |
Wensel |
| |
Wed Apr 11 |
1:15 |
Mid-Term Faculty Review - |
|
| 1 |
Wed Apr 11 |
5:15 |
TA-1 - |
|
| |
Thu Apr 12 |
1:15 |
- |
|
| |
Thu Apr 12 |
1:15 |
Mid-term Exam - |
|
| 14 |
Fri Apr 13 |
1:15 |
Structure of Lipids and Glycolipids - Major lipids of biomembranes, diversity of composition, relationships between lipid structures and functional properties |
Ludtke |
| 15 |
Mon Apr 16 |
1:15 |
Optical traps and Fluorescence Correlation Spectroscopy - |
Brownell |
| 16 |
Wed Apr 18 |
1:15 |
Membrane Protein Structure - integral and peripheral membrane proteins, protein-lipid interactions, structural features of transmembrane proteins |
Ludtke |
| 17 |
Mon Apr 23 |
1:15 |
Mass spec I - mass spectrometers, ionization methods, the use of MS in protein identification |
Malovannaya |
| 18 |
Wed Apr 25 |
1:15 |
Mass spec II - use of mass spec in protein interaction, proteomics |
Malovannaya |
| 19 |
Fri Apr 27 |
1:15 |
Post-translational modification - irreversible, lipid modifications, ubiquitination |
Weigel |
| 20 |
Mon Apr 30 |
1:15 |
Regulatory post-translational modification - Protein phosphorylation and other reversibale regulatory post-translational modification |
Weigel |
| 21 |
Wed May 02 |
1:15 |
Protein glycosylation and quality control - biosynthetic quality control, additional roles for glycosylation, analysis of glycoproteins |
Sifers |
| 2 |
Fri May 04 |
5:15 |
TA-2 - |
|
| |
Mon May 07 |
1:15 |
Faculty Review - |
|
| |
Tue May 08 |
1:15 |
Final Exam - 1:15-3:45 - |
|
| |