| The Kleberg Cytogenetics Laboratory offers fluorescence
in situ hybridization (FISH)-based assay for identifying translocations,
deletions, inversions or trisomies associated with hematopoietic disorders.
Reasons for Referral:
- Detection of specific chromosome rearrangements
- Confirmation of rearrangements difficult to detect
on karyotypes
- Assessment of minimal residual disease following treatment
or bone marrow transplant
Testing Methodology:
FISH is the application of fluorescently labeled DNA molecules
to metaphase chromosomes and interphase nuclei for the detection of chromosome
abnormalities and alterations. It is a rapid, reliable and direct approach
for diagnosis, prognosis and management of hematologic malignancies.
Hematological Malignancies Tested:
- Chronic Myeloid Leukemia (CML)
- Acute Myeloid Leukemia (AML)
- Myeloproliferative Disorders (MPD)
- Acute Lymphoblastic Leukemia (ALL)
- Lymphomas: Burkitt’s lymphoma, Hodgkin’s
disease, non-Hodgkins lymphoma (NHL)
- Lymphoproliferative Disorders (LPD): B- and T- lineage
disorders
Abnormalities
Tested:
Disease(s) |
Cytogenetic Aberrations |
Probes(Vysis) |
| AML |
translocation (8;21) |
AML1/ETO |
| AML |
translocation (15;17) and variants |
PML/RARA Dual color Dual Fusion |
| AML |
inversion (16) |
CBFB Dual Color |
| AML, ALL |
translocation (11q23) |
MLL |
| AML, ALL, CML |
translocation (9;22) |
BCR/ABL ES Dual color |
| AML, MDS |
deletion/loss 5q |
EGR1 |
| AML, MDS |
deletion/loss 7q |
D7S486 |
| AML, MDS |
deletion 20q12 |
D20S108 |
| ALL |
translocation (12;21) |
TEL/AML1 ES |
| ALL, NHL |
translocation (8;14) |
MYC Dual Color |
| CLL panel |
trisomy 12
deletion 13q14, 13q34
deletion p53 (17p13)
deletion 11q22.3
|
CEP12
D13S319,LSI13q34
p53
ATM |
| Hypereosinophilic syndrome |
deletion 4q12 |
FIPIL1-PDGFRA |
| NHL |
translocation (14;18) |
IGH/BCL2 |
| NHL |
translocation (11;14) |
IGH/CCND1 |
| NHL, MM |
translocation 14q32 |
IGH |
Bone marrow in sodium heparin (green-top) tubes:
Adult/Child: Minimum 3.0 -5.0 CC
Exception to the above are chronic granulocytic leukemia
(CGL) and chronic lymphocytic leukemia (CLL), where a blood sample can
yield adequate information. Peripheral blood is of use only when the
white blood cell count is higher than 10,000 WBC/ml and at least 10%
blasts.
All tubes must be labeled with the patient's testname and date of birth,
and a completed requisition form must accompany each sample. If the
laboratory is billing the patient's insurance, please enclose a copy
of the insurance card. Ship specimens for overnight delivery at ambient
temperature. Please notify the laboratory of incoming samples.
Turnaround Time:
5
days
Single probe: 88230, 88271, 88273, 88291
Dual probe: 88230, 88271, 88273, 88291
Multi probe: 88271x38, 88273, 88230, 88291
Forms:
>>
Cancer Cytogenetics Requisition
Test Codes:
Single probe: 8320
Dual probe: 8330
Multi probe: 8340
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