Panomics Gene Expression
Panomics, now part of Affymetrix Inc., offers gene expression with their QuantiGene Plex assay. This assay utilizes branched DNA (bDNA) and Luminex multi-analyte profiling bead (xMAP®) technology, to quantitate multiple RNA targets simultaneously, from a variety of purified or un-purified sample sources including total RNA, whole blood lysates, and FFPE tissue homogenates.
Branched DNA technology is a “sandwich nucleic acid hybridization assay” that allows for RNA detection and quantification by amplifying the reporter signal rather than the sequence, which helps avoid variation due to extraction and amplification of target sequences. The Luminex xMAP system employs flow cytometry, fluorescent-dyed microspheres, and laser detection to enable signal calculation of up to 100 unique assays within a single sample.
This assay allows researchers to detect up to 36 targets per well (96-well plate format). The simplified protocol allows for quantification without purification, reverse transcription, or amplification. Instead, mRNA is captured to the respective microspheres via the “Capture Probe” and “Capture Extender” interface. The mRNA is then hybridized to a bDNA amplifier and Biotin-SAPE labeling agents, in succession. The fluorescence is then read on the Luminex 200 Scanner.
| Total RNA Input | Concentration |
|
11-300 ng
|
0.55-15 ng/µL
|
*Dilution series must be performed to determine optimal RNA input amount
Please visit the Panomics website for assay formats and custom design options.
NOTE: All samples must pass GARP Sample Quality Control (SAQC) before they can be submitted for assay processing.