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Cytometry and Cell Sorting Core

Houston, Texas

BCM is ranked No. 1 for research expenditures in biological science by the National Science Foundation.
Cytometry and Cell Sorting
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Assisted Fluorescence-Activated Cell Sorting

General Information

We offer operator-assisted Fluorescent-Activated Cell Sorting. This service is available Monday through Friday from 10 a.m. to 6 p.m.

How to Request Assisted Sorting Appointments

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Sorting Equipment and Features

Both of our Fluorescence-Activated Cell Sorters have the newest technology including the following equipment features:

  • Sorting into Plates, slides or tubes
  • Sort up to 25,000 events/second
  • Temperature- controlled sample chamber and collection chamber
  • Up to 14 colors plus forward and side scatter simultaneously allowing complex gating strategies
  • Up to Four-way tube sorting (for small, 5mL or 1.5mL, tubes only)
  • Viewable sorting graphic display
  • Plate sorting- Any number of cells can be deposited into any number of wells (including single-cell deposition)
  • Complementary computer print-outs or analysis results, sort reports, and electronic data
  • Ultra-sensitive Forward-Scatter PMT Detector Upgrade for small-particle discrimination
  • Ultra-sensitive Forward-Scatter PMT Detector Upgrade for small-particle discrimination

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FACSAria - three laser

- Blue (488nm) laser [e.g.FITC, PE, PE-Cy5, PE-Cy7, PerCP, Alexa488, GFP, YFP, Sytox Blue]
- UV (355nm) laser [e.g. Hoechst, DAPI]
- Red (638nm) laser [e.g. APC, APC-Cy5, APC-Cy7, Alexa647, Sytox Red]

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FACSAriaII - four laser

- Blue (488nm) laser [e.g. FITC, Alexa488, GFP, YFP, Sytox Blue]
- UV (355nm) laser [e.g. Hoechst, DAPI]
- Red (638nm) laser [e.g. APC, APC-Cy5, APC-Cy7, Alexa647, Sytox Red]
- Yellow-Green (561nm) laser [e.g. RFP, mCherry, dTomato, dsRed, PE, PE-Cy5, PE-Cy7, PerCP]

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Sorting and Sample Prep Guidelines

Please download and read our sorting guidelines to help you better prepare yourself for a sort appointment.

Download the Sorting and Sample Prep Guideline (pdf) for detailed information.

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Sorting Fees and Cancellations

Fees

  • Assisted Sorting Charge: $90/hour (Minimum 1 hour)
  • Sort Set-Up Fee: $25

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Cancellations

Please give 24-hours notice for all cancellations to operator-assisted appointments. For more information please see our Billing Policies

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