| Service |
Description |
Cost |
| Electroporation |
Insertion of a single construct into one cell line. The core will pick a minimum of 200 and maximum of 300 colonies for one electroporation. |
$3,300 |
| ROSA Targeting |
Insertion of a single ROSA construct into one cell line. The core will pick a total of 24 colonies. |
$500 |
| Clone Expansion |
After Mini Southern confirmation data is gathered by investigators the core will expand positives clones from one well of a 96 well plate to one 100 mm plate and freeze two vials for LN2 long term storage. |
$120/per clone |
| Generation of Primary Mouse ES Cell Lines |
Generation of a total of three independent mouse ES cell lines from three independent embryos. Investigators will provide plugged females to the core. |
$1,800 |
