Fig 1. (Left) A neuron from a midbrain VTA slice that was electrically monitored was back-filled with biocytin and was visualized with fluorescein-conjugated avidin. (Middle) The same neuron (*) and a nearby neuron were labeled with a monoclonal anti-TH antibody that was visualized with a Texas Red-conjugated IgG, indicating this was a DA neuron from the VTA. (Right) The Ih current is indicative of DA neurons from the midbrain.

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