Cell Based Assay Screening Service
The C-BASS core makes available to the BCM community the human and mouse shRNA library collections, through the Open Access Program from Open Biosystems. C-BASS can provide individual shRNA plasmids for use in any BCM laboratory, or carry out an entire screening experiment using the libraries.
C-BASS personnel are available to facilitate the use of these reagents and perform RNAi screens with a level of involvement that is appropriate for the specific experiment. Downstream analyses of the RNAi screen can include, in-house, high-throughput image analysis and flow cytometry. In addition, DNA oligo chip hybridization for the identification of the barcoded shRNA plasmids can be carried out at the BCM Microarray Core Facility.
Introduction to Services and Resources
For pricing information please see the C-BASS Price Schedule.
For reservations of equipment use, including IMXMicro, IMX Ultra, Biomek automated workstations (FX, NX, 3K), and LSRII with HTS, please go to http://www.cbass.bcm.tmc.edu/Reservations/. Register first if you are a new user. You will receive email notifications once reservations are complete.
For all orders and services other than obtaining individual shRNA clones, please e-mail c_bass@bcm.edu or call 713-798-8987.
Clone distribution **The shRNA reagents are available to BCM researchers ONLY.
The core distributes the human and mouse shRNA library collections from Open Biosystems for a nominal fee. Each clone – in glycerol stocks – costs $25; orders are normally processed each Wednesday. Orders placed before 5pm on Tuesday will be processed for that week. Rush Service Now Available! See our Ordering page for more information.
Print a copy of our Ordering page to assist your navigation and account set up within Open Biosystems. Log into Open Biosystems.
Positive control clones (GAPDH and EG5) and negative control clones (non-silencing and empty vector) are also available for $25 each.
Target-based shRNA libraries or druggable genome collections
Kinome and Phosphatome sub-libraries are now available.
C-BASS anticipates great interests in sub-library collections that are tailored specifically to the interests of the individual investigator. C-BASS is equipped to selectively array a small collection of shRNAs based on your preferences. Examples include shRNAs against a particular signaling pathway or activity. These collections should prove especially attractive to investigators who are more interested in genetically probing the function of a definable set of targets.
shRNA-expressing cell line(s) and Lentiviral Stocks
The C-BASS core will establish the cell line of interest (to be provided by the user) that expresses the shRNA(s) of interest (from the shRNA collection). Lentivirus can be made by the C-BASS core for any of the shRNA or a construct of your interest for $250. Discount for bulk orders is available.
Discounts on shRNA related products from Open Biosystems
Openbiosystens offers discounts for certain shRNA related products. View a list of shRNA related products from Open Biosystems, and contact Open Biosystems at info@openbiosystems to inquire about promotional discounts.
DNA purification
Facilitated by Beckman Coulter robotics systems, the core will purify DNA in a high throughput manner. 96 well plates will be purified and the OD taken to ensure quality. Contact us for more information.
Flowcytometer
The C-BASS core will allow for usage of its BD Biosciences LSRII flowcytometer utalizing only the HTS (high throughput system) for 96 well applications. For more information concerning usage of the LSRII please contact C-BASS.
Automated Microscopy
The C-BASS core will allow for usage of its automated microscopes for high-throughput imaging.