Human Tissue Acquisition and Pathology
(Thomas Wheeler, M.D., Director;
Pamela Younes, Co-Director)
Services:
Tissue Acquisition and Distribution
Supported by the The Dan L. Duncan Cancer Center, human tissue banking activities have been expanded to include development of a uniform patient consent and standardized tissue collection processes across all tumor sites at the Michael E. DeBakey Veteran’s Administration Hospital and Ben Taub General Hospital.
Description
- Distribution of banked tissue specimens to investigators
- Project-based fresh tissue collection
- Clinical trial support of tissue acquisition effort
- Inventory and data management
Archive, Tissue Microarray (TMA) and Research Services
The Pathology tissue bank manages an archive that contains formalin fixed paraffin-embedded tissue specimens from a variety of organ/disease types. Approximately 10 to12 years of clinical, pathological and follow-up data is available for most of these tissue specimens. Using this valuable collection and our TMA expertise, we also offer TMA services.
Description
- Search: Identify the cases that meet investigator’s project need.
- TMA design: TMA is designed to address questions in investigator’s proposal. Clinical & pathological data that is associated to each specimen on the array is displayed in the design in an excel format.
- TMA and cell array construction: TMA and cell array can be built using specimens provided by the investigator. TMA can also be built using our archival tissues.
- TMA quality control: Tissue/cell type and diagnosis for each specimen on TMA is confirmed by a pathologist. Clinical follow up is available for most of these specimens via the hospital cancer registry.
Histology and Pathology Services
Description
- H&E-staining and histochemistry
- Unstained tissue/tissue array sectioning
- Immunohistochemistry (IHC) using either manual or automated DAKO immunostainer
- Apoptosis (TUNEL) assays
- Tissue/cell processing and embedding
- Sectioning of thick tissue curls for RNA/DNA isolations
- Pixell II laser capture microdissection
- Scoring IHC and TUNEL results
- Photography, digital
- Florescent/Confocal microscopy
Circulating Tumor Cell (CTC) Analysis (CellSearch™ System)
The CellSearch™ System offers an advanced, semi-automated assay that enables the capture, identification, and classification of rare circulating tumor cell (CTCs) in a standardized, objective manner. Combining high accuracy, specificity, and reproducibility with state-of-the-art automation, the CellSearch™ System is the only laboratory platform that standardizes sample collection, cell capture, staining, enumeration, and characterization of CTCs. The CellSearch™ System consists of a Autoprep™ system and Analyzer II. Blood is collected and processed through an immuno-magnetic methodology to obtain, enumerate and classify specific markers for breast, colorectal and prostrate cancer.
Description
- Enumerating circulating tumor cells (CTC) in blood.
- Enumerating circulating endothelial cells (CEC) in blood.
- Profiling tumor cells by using available assays for expression of known biomarkers (MUC1, EGFR, and HER2).
- Help investigators develop expression profile assays of CTC biomarkers of interest.
- Enrich cells via immunomagentic processes for investigator(s) to perform further studies offline (e.g. molecular assays, FISH, flow cytometry)
Charge Back Fees
SERVICE |
DESCRIPTION |
PRICE |
UNIT |
|
NON-CANCER CENTER MEMBER |
CANCER CENTER MEMBER |
|||
| Fresh Tissue | Specimen collection only | $30 |
$20 |
Per specimen |
| Specimen, clinical data & pathology report | $40 |
$25 |
Per specimen | |
| Frozen Tissue (FF) | Specimen pull & delivery | $40 |
$30 |
Per specimen |
| Specimen, one H&E, diagnosis confirmation | $50 |
$35 |
Per specimen | |
| Specimen, one H&E, diagnosis confirmation, clinical data & pathology report | $60 |
$40 |
Per specimen | |
Unstained tissue sections, 4-5 µm, one H&E, diagnosis confirmation, clinical data & pathology report |
$40 |
$30 |
5 unstained tissue sections |
|
$50 |
$35 |
10 unstained tissue sections | ||
Tissue curls, 10 µm per curl in a 1.5 ml enpendorf tube, one H&E, diagnosis confirmation, clinical data & pathology report |
$40 |
$30 |
5 tissue curls, 10 µm Per curl |
|
$50 |
$35 |
10 tissue curls, 10 µm Per curl | ||
| Formalin-Fixed Paraffin-Embedded Tissue (FFPE) | Specimen pull & delivery | $20 |
$10 |
Per specimen |
| Specimen, one H&E, diagnosis confirmation | $30 |
$15 |
Per specimen | |
| Specimen, one H&E, diagnosis confirmation, clinical data & pathology report | $40 |
$20 |
Per specimen | |
Unstained tissue sections, 4-5 µm, one H&E, diagnosis confirmation, clinical data & pathology report |
$20 |
$10 |
5 unstained tissue sections | |
$30 |
$15 |
10 unstained tissue sections | ||
Tissue curls, 10 µm per curl in a 1.5 ml enpendorf tube, one H&E, diagnosis confirmation, clinical data & pathology report |
$20 |
$10 |
5 tissue curls, 10 µm Per curl |
|
$30 |
$15 |
10 tissue curls, 10 µm Per curl | ||
| Tissue Microarray (TMA) | TMA construction using tissue/cell blocks from investigator |
$125 |
$100 |
30 cores, 0.6mm |
$250 |
$200 |
50 cores, 0.6mm | ||
$500 |
$400 |
100 cores, 0.6mm | ||
$875 |
$700 |
200 cores, 0.6mm | ||
$1,625 |
$1,300 |
360 cores, 0.6mm | ||
$875 |
$700 |
80 cores, 1.0mm | ||
$625 |
$500 |
42 cores, 2.0mm | ||
| TMA unstained slide using archived FFPE blocks | $50 to $195 |
$30 to $150 |
Per unstained slide depend on number of cases, and size of cores on the array | |
| Processing & Embedding | Formalin fixed & paraffin embedded tissue block | $6 |
$4 |
Per block |
| OCT tissue block | $6 |
$4 |
Per block | |
| Snap frozen tissue block | $6 |
$4 |
Per block | |
| Tissue decalcification | $6 |
$4 |
Per sample | |
| Tissue re-embedding | $2 |
$1 |
Per block | |
| Formalin fixed & paraffin embedded cell block | $7 |
$5 |
Per sample | |
| OCT cell block | $7 |
$5 |
Per block | |
| Hand Processing | $15 |
$10 |
Per block | |
| Microtome | Frozen tissue sectioning | $8 |
$5 |
Per slide |
| Paraffin tissue sectioning | $5 |
$3 |
Per slide | |
| Preparation of tissue slices for RNA/DNA retrieval | $8 |
$5 |
10 µm Per slide, 5 slides Per block |
|
| TMA sectioning | $8 |
$5 |
Per slide | |
| Cytospin | $5 |
$3 |
Per slide | |
| Staining | H&E staining | $5 |
$3 |
Per slide |
| IHC Titration | $150 |
$100 |
Per antibody | |
| Staining using investigator supplied antibody | $15 |
$10 |
Per slide | |
| IHC using core sullied antibody | $18 |
$12 |
Per slide | |
| Double immuno staining | $45 |
$30 |
Per slide | |
| Cytology smears stained | $5 |
$3 |
Per slide | |
| Special Stain-Group I (simple, non silver) |
$12 |
$7 |
Per slide | |
| Special Stain-Group II (complex, silver) | $15 |
$9 |
Per slide | |
| Microscopy & Pathology Evaluation | Pixell II laser capture micro dissector | $100 |
$50 |
Per hour |
| Scoring IHC results | $75 |
$50 |
Per hour | |
| Photography, digital | $75 |
$50 |
Per hour | |
| Florescent/Confocal microscopy | $75 |
$50 |
Per hour | |
Circulating Tumor Cell Analysis |
CTC enumeration | $250 |
$200 |
Per sample |
| CEC enumeration | $250 |
$200 |
Per sample | |
| Epithelial Cell Enrichment | $120 |
$80 |
Per sample | |
| CTC Phenotyping (MUC1, EGFR, HER2) |
Contact for Pricing |
|||
| CTC Phenotyping (custom assay) |
Contact for Pricing |
|||
Locations:
- Tissue Acquisition and Distribution
BCM Department of Pathology
Room 220B
(Pamela S. Younes, M.S., B.A.)
- Archive, TMA and Research Services
BCM Department of Pathology
Veterans Affairs Medical Center, 109 Room 118
(Xiaoyun Shen, M.D., Ph.D.)
- Histology and Pathology Research Services
These services are conducted at the VA Medical Center. There is a drop-off location at Baylor College of Medicine for daily pick-up of samples (BCM 286A)
(Xiaoyun Shen, M.D., Ph.D.)
- CellSearchTM System
BCM Department of Pathology
Room 209E
(See Dr. Tom Wheeler or Pamela Younes)
Director:
Thomas M. Wheeler, M.D.
Professor and Chair, Department of Pathology
Professor, Department of Urology
Phone: 713-798-4664
E-mail: twheeler@bcm.edu
Personnel:
Pamela Younes, M.S., B.A.
Co-Director
Phone: 713-798-5581
E-mail: pyounes@bcm.edu
Xiaoyun Shen, M.D., Ph.D.
Manager
Archive, TMA and Research Services
Phone: 713-794-8296
E-mail: xshen@bcm.edu
Dandan He, M.D.
Archive, TMA and Patient Consent
Phone: 713-794-8296
Email: dandanh@bcm.edu