Flow Cytometry Shared Resource
(Joel M. Sederstrom, MS, Technical Director, Ellen A. Lumpkin, Ph.D., Academic Director)
The Dan L Duncan Cancer Center Flow Cytometry Shared Resource combines the resources of the BCM Cytometry and Cell Sorting Core and the Texas Childrens Hospital Flow Cytometry Core.
Services at Baylor College of Medicine
Services at Texas Children's Hospital
Baylor College of Medicine’s Cytometry and Cell Sorting Core Facility
The new Cytometry and Cell Sorting Core Facility at Baylor College of Medicine provides instrumentation and advice for a wide variety of flow cytometry and cell sorting needs. The core supplies the BD LSRII, a four-laser flow cytometer, that allows researchers to analyze up to 16 different fluorochromes plus forward and side scatter. The LSRII’s High Throughput System (HTS) allows numerous small volume samples to be run automatically and can be used with multi-well plates. The core also provides two cell sorters that can sort up to four different populations or single cells into a multi-well plate. The newest of which is a BD FACS AriaII that has four lasers and can sort samples using 13 fluorochromes in addition to forward and side scatter. There is also a BD FACS Aria that has three lasers and can sort samples using 14 parameters. The core’s Miltenyi AutoMACS is an automated magnetic cell sorter equipped with multiple settings including ones for sensitive cells, increased cell purities or increased cell yields. There is also a Beckman-Coulter Vi-CELL that uses trypan blue exclusion to automatically stain, count, assay cell size and viability for up to twelve samples at a time. The facility provides multiple workstations available with the latest software for data analysis as well as a dedicated server for storage. In addition to equipment the facility provides flow cytometry consultations and instrument training including sample preparation, analysis and cell sorting.
Cytometry and Cell Sorting Facility at Baylor College of Medicine
Location:
Cytometry and Cell Sorting Core Facilty
Taub 103
Baylor College of Medicine
One Baylor Plaza
Houston, TX 77030-3411
Contact Information:
Phone: 713-798-3868
Fax: 713-798-9009
Joel M. Sederstrom, MS
Director of Flow Cytometry
Dan L. Duncan Cancer Center
Office of Research
Phone: 713-798-3774
E-mail: sederstr@bcm.edu
Services at Texas Children's Hospital
The Flow Cytometry Core Unit is divided into three sections: basic and clinical research, patient and sample monitoring for the Bone Marrow Transplant group and clinical diagnostics for the Hematology / Oncology Clinic. We currently have five flow cytometers (2 cells sorters and 3 cell analyzers) and three personnel in the core unit and we deal with a variety of different projects for many different labs. The Cytomation MoFlo is our sorting cytometer. We use it to separate populations of cells from one another by means of fluorescently labeled antibodies and/or DNA dyes. We can enrich populations up to 4 log while maintaining viability and sterility. One Cytomation MoFlo multi-laser cell sorter for research is equipped with three lasers and is capable of analyzing 8 colors and 2 size parameters (relative size and density). It can sort up to four populations at a rate of >25,000 cells/second. We have a similar cell sorter for clinical use within the GMP facility that is equipped with two lasers and can analyze using 7 parameters and sort one or two populations.
The other part of our cytometry core performs cellular analysis. We have three machines used for analyzing cells, detecting up to four colors and two size parameters. There is a three-color Becton Dickinson FACScan within the GMP for patient and sample monitoring, and one in our research core used for both basic research and clinical diagnostics. Our third analyzer is a Becton Dickinson FACSCalibur, which is used for basic and clinical research and is capable of four-color analysis.
The Core provides expert cell analysis and sorting as well as data management and archival, advanced computer modeling of data, sample preparation methodology and a broad supply of knowledge and experience in the field of cytometry. We are constantly working to push the limit of the technology available and help devise new technologies and applications related to flow cytometry.
Flow Cytometry Core Laboratory at Texas Children's Cancer Center
Location:
Flow Cytometry Laboratory
Feigin Center 10th Fl, Ste 1025.16
Texas Children's Hospital
1102 Bates St.
Houston, TX 77030
Contact Information:
Phone: 832-824-4731
Fax: 832-825-4846
Laboratory Manager:
Mike Cubbage
Office: 832-824-4252
Email: mlcubbag@txccc.org